Long-term storage of carbon in the terrestrial biosphere, belowground, ocean and soil, which helps in decreasing the carbon-dioxide (the principal greenhouse gas) concentration in the atmosphere, is termed as carbon sequestration. Carbon sequestration can be enhanced throughthe expansion of forest and management of degraded lands. Assessment of plant and soil carbon sequestered in any forest is important as it gives ecological, environmental and economic benefits to the people. The focal point of the study was to assess the net carbon sequestration for Dendrocalamus. Sigana VDC ward No.-2 from Baglung district was selected for this study. Simple random sampling was carried out for collecting the biophysical data for estimating above and below ground biomass of bamboo. Diameter at breast height (Dbh) was measured at 15cm and height was measured with tape and sunto clinometer. One bamboo from each plot was felled and samples were taken for determining the carbon content of bamboo culms and leaves. Twelve profiles were dug out for determining the soil organic carbon. The soil samples were taken from soil profile up to bed rock or maximum of 1m depth at the interval of 20 cm. The bamboo samples were burnt at 400oC for half an hour in muffle furnace to calculate carbon content of bamboo shoots and leaves. Likewise, bulk density and soil organic carbon were obtained from the soil samples in the laboratory. Also demand and supply and uses of bamboo were assessed. Laboratory analysis of culms and leaves for carbon content showed 33.26% & 19.53% carbon content in culms and leaves, respectively. The above and below ground biomass carbon sequestration in bamboo was found as 1.66 t/ha and 0.08t/ha., respectively, whereas the soil carbon sequestration was found to be 230.32 t /ha. Finally, the total carbon sequestration in bamboo forest was found to be 232.06 tons/hectare. The demand and supply was found to be 1600 culms per household and 2600 culms per household, respectively. Similarly, the bamboo was used in bioengineering, fodder, fuelwood, food, fencing, making bamboo products like doko, muda etc., and props for house construction purposes.